Before you arrive at lab, please do the following:
Read the Standard Operating Procedures for the EM Lab. Complete the online Right to Know safety module at UGA Env. Safety Division and bring the certificate to class.
Please read Bruce Railsback's Comments on ethical issues of research.
Download the Course Syllabus
Download the EM Lab manual (pdf)
REFERENCES:
To access the e-version of the book "Electron Microscopy" by Bozzola and Russell go to the UGA library site (
http://www.libs.uga.edu/), and click on Galileo Database button. Search for author "bozzola" and it should provide several options, one of which is the Electron Microscopy textbook. Choose the e-version and it will pop up for you. You may need to "electronically" check out the book if you are spending too much time or flipping through several pages.
The other text that will be helpful is available here as a PDF. It's called "Microscopy: Basics and Beyond" by Mortimer Abramowitz. This document will provide information on light and fluorescence microscopy.
Two PDFs are available from Oxford Instruments explaining EDS and WDS for x-ray analysis.
As the class progresses, we will post helpful links to other sites below that will assist in your course work. Some may be used for illustrating concepts in lecture.
LINKS:
Electron Microscopy
Electron Microscopy for Dummies - http://www.snaggledworks.com/em_for_dummies/
TEM Basics - http://www.matter.org.uk/tem/default.htm
Some basic EM techniques (neg staining, immuno labeling, etc...) - http://www.urmc.rochester.edu/research/emimg/techniqu.html
Negative Staining information - http://www.emlab.ubc.ca/Documents/Negative_Staining.htm
Online EM preparation protocols - http://www.protocol-online.org/prot/Image_Techniques/Microscopy/Electron_Microscopy/
Light and Laser Microscopy
Molecular Expressions Optical Microscopy Primer - http://micro.magnet.fsu.edu/primer/index.html
Basic light microscopy and associated techniques - http://www.ruf.rice.edu/~bioslabs/methods/microscopy/microscopy.html
The Teaching Modules at EMBL (EAMNET) - covers general history, FRET, FRAP and other analytical microscopy methods.
How to give a presentation and general talks on light and confocal microscopy.
Nikon MicroscopyU - http://www.microscopyu.com/
Laser Basics tutorial - http://members.aol.com/WSRNet/tut/ut1.htm
Glossary of Laser Basics - http://www.columbia.edu/cu/mechanical/mrl/ntm/Glossary.html
Fluorescent Dye spectra (interactive) - http://www.mcb.arizona.edu/IPC/spectra_page.htm
MicroCT (x-ray tomography)
Skyscan product site - http://www.skyscan.be/next/home.htm
Toshiba MicroCT with how it works - http://www.toshiba-itc.com/cat/en/prod02.html
For more information about the course, please contact Dr. Mark Farmer(farmer@cb.uga.edu) or Dr. John Shields (jpshield@uga.edu) or call the EM Lab on UGA campus (706-542-4080)
