TEM INDEX

TEM Notes #1

Brief History of TEM
Introduction to TEM
Biological Specimen Preperation

• Goals
  
• Problems
  

Fixation

• Buffers
  • Cacodylate
  • Collidine
  • HEPES
  • Phosphate
  • PIPES
  • Tris
  • Veronal Acetate
• Fixatives
  • Formaldehyde
  • Gluteraldehyde
  • Acrolein
  • Osmium Tetroxide
  • Permanganate
  • Diimdidoesters
• Methods of Fixation
• Dehydration
• Embedding Media
  • Methacrylates
  • Polyester Resins
  • Epoxy Resins
  • Alternative Embedding Media
    • 10% Polystyrene
    • LR White
    • LR Gold
    • Lowicryl
    • Polyethylene Glycols

Support Films
Sectioning

• Problems
  • Resin Polymerization
  • Block Face
  • Equipment
• Knives
• Sectioning Imperfections
  • Knife Marks
  • Compression
  • Chatter
• Section Thickness
• Pick Up Sections
• Staining Sections
  • Positive Staining
  • Post-embedding Staining

Theory Of Electron Optics

• Resolution
• Electromagnetic Lenses
• Electrostaic Lenes

TEM Lenses

• Condensor
• Objective
• Projector

Vacuum Systems

• Mechanical Pump
• Diffusion Pump
• Sputter-ion Pump
• Orbitron Pump
• Turbomolecular Pump
• Cryogenic Pump

Autoradiography
Electron Energy Loss Spectrscopy (EELS)
Electron Spectroscopic Imaging (ESI)

TEM Notes #2

Negative Staining

• Advantages
• Disadvantages
• General Procedure

Replicas and Shadowing

• Shadowing
  • Heated Electrodes
  • Electron Beam Evaporation
  • Cathode Etching
• Shadow Cast

Cryopreservation

• Rapid Freezing
• Cryoprotectants
• Freeze Drying and Distillation
• Cryosectioning
  • Freeze Fracture

Immunoelectron Microscopy
Enzyme Cytochemistry
Lectins
Stereology
X-Ray Microanalysis

• EDX
• WDS

Electron Diffraction
Intermediate and High Voltage EM