| Please cross reference with our SEM notes, TEM notes and the class textbook (Bozzola and Russell)
Overview of specimen preparation
Factors affecting specimen preparation
Fixation overview
Fixatives
Buffers
En Bloc Stains
Overview of special preparation techniques and labeling |
PowerPoint Slides from Lecture
Safety in an EM Lab
History of EM
Ultrastructure of Cells
Specimen Preparation (Buffers, Fixatives)
Dehydration, embedding, sectioning
Section mounting, support films, post-staining
SEM mounting, drying, coating
Shadow coating, Replicas
Variable Pressure and Environmental SEM
Cryofixation for TEM and SEM
Autoradiography
Immunocytochemistry
Labeling - Lectins, enzymes, etc...
Overview of some EM artifacts
Confocal Microscopy
Focused Ion Beam/dual beam
Tomography
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