BIOL/CBIO 5040L/7040L
Electron Microscopy Laboratory
Drs. Mark Farmer & John Shields

August 22    Introduction to EM Facility/Laboratory Safety and Specimen Fixation

24    Fixation, dehydration, and embedding

29    Knife making and block trimming

31    Sectioning   [INDIVIDUAL PROJECT PROPOSALS DUE]

Sept. 5    Grid Staining

7    Basic operation of JEOL 100CX TEM

12    Support Films

14    Operation of JEOL 100 CX Continued

19    Negative Staining   [SECTIONS ON GRIDS DUE]

21    JEOL 100 CX - Camera Exchange and Development

26    Specimen Fixation for SEM

28    Critical Point Drying and Mounting

Oct. 3    Specimen Coating for SEM

5    JEOL 100 CX TEM - Check out Examinations

10    Introduction to JEOL SEM (Veterinary School EM Facility)

12    JEOL SEM Continued

17    Darkroom and Photography

19    Specimen Preparation for X-ray and carbon coating

24    Backscatter and X-ray (LEO 982 SEM)   [LEAF IMAGES DUE]

26    FALL BREAK

31    Introduction to LEO 982 SEM

Nov. 2    Oxford X-ray System   [TEM PRINTS DUE]

7    X-ray Projects (Graduate Students)

9    Shadowcasts and Replicas

14    Electron Diffraction

16    Electron Spectroscopic Imaging and EELS (Zeiss 902 TEM)

21    Cryopreservation & Freeze Fracture

23    THANKSGIVING HOLIDAY

28    Immuno-EM   [INDIVIDUAL PROJECT NEGATIVES DUE]

30    Digital Imaging Laboratory

Dec. 5    Confocal Microscopy

7    Critique

   [COMPLETED PROJECTS* DUE AT TIME OF FINAL EXAMINATION]

        *Includes X-ray portion for graduate students


GO to Lecture Syllabus
GO to Lecture Notes