BIOL/CBIO 5040L/7040L
Electron Microscopy Laboratory
Drs. Mark Farmer & John Shields
August 22 Introduction to EM Facility/Laboratory Safety and Specimen Fixation
24 Fixation, dehydration, and embedding
29 Knife making and block trimming
31 Sectioning [INDIVIDUAL PROJECT PROPOSALS DUE]
Sept. 5 Grid Staining
7 Basic operation of JEOL 100CX TEM
12 Support Films
14 Operation of JEOL 100 CX Continued
19 Negative Staining [SECTIONS ON GRIDS DUE]
21 JEOL 100 CX - Camera Exchange and Development
26 Specimen Fixation for SEM
28 Critical Point Drying and Mounting
Oct. 3 Specimen Coating for SEM
5 JEOL 100 CX TEM - Check out Examinations
10 Introduction to JEOL SEM (Veterinary School EM Facility)
12 JEOL SEM Continued
17 Darkroom and Photography
19 Specimen Preparation for X-ray and carbon coating
24 Backscatter and X-ray (LEO 982 SEM) [LEAF IMAGES DUE]
26 FALL BREAK
31 Introduction to LEO 982 SEM
Nov. 2 Oxford X-ray System [TEM PRINTS DUE]
7 X-ray Projects (Graduate Students)
9 Shadowcasts and Replicas
14 Electron Diffraction
16 Electron Spectroscopic Imaging and EELS (Zeiss 902 TEM)
21 Cryopreservation & Freeze Fracture
23 THANKSGIVING HOLIDAY
28 Immuno-EM [INDIVIDUAL PROJECT NEGATIVES DUE]
30 Digital Imaging Laboratory
Dec. 5 Confocal Microscopy
7 Critique
[COMPLETED PROJECTS* DUE AT TIME OF FINAL EXAMINATION]
*Includes X-ray portion for graduate students